Which laboratory tests are essential for diagnosing autoimmune thyroiditis in primary care?

Guideline-aligned answer with reasoning, red flags and references. Clinically reviewed by Dr Kola Tytler MBBS CertHE MBA MRCGP.

Posted: 17 August 2025 Guideline-Aligned (High Confidence) Clinically Reviewed
Dr Kola Tytler MBBS CertHE MBA MRCGPClinical Lead • iatroX

For diagnosing autoimmune thyroiditis in primary care, the essential laboratory tests typically involve assessing thyroid function and specific antibodies:

  • Initial Thyroid Function Tests (TFTs):
    • For adults where secondary thyroid dysfunction (pituitary disease) is not suspected, consider measuring thyroid-stimulating hormone (TSH) alone . If the TSH is above the reference range, free thyroxine (FT4) should be measured from the same sample .
    • For children and young people, or adults where secondary thyroid dysfunction is suspected, consider measuring both TSH and FT4 .
    • These tests may be repeated if symptoms worsen or new symptoms develop, but no sooner than 6 weeks from the most recent test .
  • Thyroid Peroxidase Antibodies (TPOAbs):
    • To identify autoimmune thyroiditis, consider measuring thyroid peroxidase antibodies (TPOAbs) for adults with TSH levels above the reference range . TPOAbs testing should not be repeated .
    • For children and young people with TSH levels above the reference range, TPOAbs should be measured .
    • Positive TPOAbs indicate an increased risk of primary hypothyroidism . They are also associated with an increased risk of a more severe episode of postpartum thyroiditis and a higher likelihood of postpartum hypothyroidism .

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