A 68-year-old woman presents to the Emergency Department feeling generally unwell with a productive cough and fever for 2 days. Her observations are: Temp 38.5°C, HR 115 bpm, RR 24/min, BP 95/55 mmHg, SpO2 92% on air. Her lactate is 2.8 mmol/L. According to the Sepsis Six pathway, which of the following interventions should be completed within one hour of recognition of sepsis?